Solid phase microextraction in medical and translational research

15 September
09:00 – 13:00 (4 hours)
Provided by: Prof. Janusz Pawlyszyn and Prof. Barbara Bojko, Waterloo University, Canada

In the course participants will learn about applications of solid phase microextraction to medical research, clinical practice and drug discovery and development. The high throughput and automation for in vitro and ex vivo study of large as well as small sample volumes i.e. spot sampling, cell line study will be discussed. We will underline how users can benefit from certain features of various device geometries, shapes, sizes and coatings when developing methods for different applications. Low invasive in vivo and in situ organ sampling in animals and in humans in the view of pharmacokinetics, therapeutic drug monitoring and biomarkers discovery will be explained on several examples. Practical approaches to quantitation with the use of kinetic and equilibrium-based calibration will be clarified. Also, recent advances in untargeted metabolomics and lipidomics will be shown with particular focus on applications to oncology, neuroscience and transplantology.

The course is targeted at both future and current SPME users, who wish to gain a deeper insight into the technology utilized in bioanalysis and thus improve data quality and increase their productivity.

  • PhD students
  • Analytical chemist
  • Biologists, clinicians and bioanalytical scientists
  • Laboratory supervisors
  • Scientists and industry regulators
Janusz

Professor, Pawliszyn Janusz

The primary focus of Professor Pawliszyn's research program is the design of highly automated and integrated instrumentation for the isolation of analytes from complex matrices and the subsequent separation, identification and determination of these species. He is an author of over 550 scientific publications and a book on Solid Phase Microextraction. His Hirsch Index (H-index) is 88. He is a Fellow of Royal Society of Canada, editor of Analytica Chimica Acta, Trends in Analytical Chemistry and a member of the Editorial Board of Journal of Separation Science and Journal of Pharmaceutical Analysis. He initiated a conference, “ExTech”, focusing on new advances in sample preparation and disseminates new scientific developments in the area, which meets every year in different part of the world. He received the 1995 McBryde Medal, the 1996 Tswett Medal, the 1996 Hyphenated Techniques in Chromatography Award, the 1996 Caledon Award, the Jubilee Medal 1998 from the Chromatographic Society, U.K., the 2000 Maxxam Award from Canadian Society for Chemistry, the 2000 Varian Lecture Award from Carleton University, the Alumni Achievement Award for 2000 from Southern Illinois University, the Humboldt Research Award for 2001, 2002 COLACRO Medal, 2003 Canada Research Chair, in 2006 he has been elected to the most cited chemists by ISI, in 2008 he received A.A. Benedetti-Pichler Award from Eastern Analytical Symposium, 2008 Andrzej Waksmundzki Medal from Polish Academy of Sciences, 2008 Manning Principal Award, 2010 Torbern Bergman Medal from the Swedish Chemical Society, 2010 Ontario Premier’s Innovation Award, 2010 Marcel Golay Award, 2010 ACS Award in Separation Science and Technology, 2011 PittCon Dal Nogare Award, 2012 E.W.R. Steacie Award, 2013 Environmental Research and Development Award and 2013 LeSueur Memorial Award, 2015 Maria Skłodowska-Curie Medal from Polish Chemical Society, 2015 Halász Medal Award from the Hungarian Society for Separation Sciences, 2017 Pittsburgh Conference Analytical Chemistry Award, the 2017 Eastern Analytical Symposium Award for Outstanding Achievements in the Fields of Analytical Chemistry, 2018 ACS Award in Chromatography and 2018 North American Chemical Residue Workshop Excellence Award.. He presently holds the University Professor, Canada Research Chair and Natural Sciences and Engineering Research Council of Canada Industrial Research Chair in New Analytical Methods and Technologies.

Barbara Bojko

Barbara Bojko, Ph.D., D.Sc.

Scientific career
Barbara Bojko studied laboratory medicine at the Medical University of Silesia (Poland), where she received her Master’s degree in 2001 and Ph.D. degree in pharmaceutical sciences in 2005 with specialization in pharmaceutical biochemistry. The same year she became an Assistant Professor at the same university and in 2009 she joined Prof. Pawliszyn group at University of Waterloo (Canada), initially as a Postdoctoral Fellow and later as a Research Associate (2012-2015). Among the grants she coordinated were those sponsored by the World Anti-Doping Agency (WADA); Heart and Stroke Foundation of Canada; CQDM, Brain Canada and Ontario Brain Institute; Canadian Institutes of Health Research (CIHR) and Natural Sciences and Engineering Research Council of Canada (NSERC). In 2014, she completed her Habilitation (D.Sc.) in the field of analytical chemistry at Medical University of Gdańsk (Poland) and became an Associate Professor at Nicolaus Copernicus University (Poland), where she established her research group. Dr. Bojko is an editorial board member of International Journal of Analytical Chemistry, MethodsX and Medical Research Journal. She was also one of the co-founders of Polish Metabolomics Society, where she currently holds a position of Secretary of the Society.

Research interest
Initially her studies involved various aspects of ligand-protein binding i.e. drug-protein interaction, fatty acid-protein interaction, drug-drug and drug-fatty acid competition for protein binding. Currently, her research focuses mainly on the introduction of new analytical solutions based on microextraction techniques and mass spectrometry to clinical diagnostics and research in the field of pharmaceutical sciences. Her particular interested is in low- and non-invasive tissue analysis in the field of oncology, transplantology, and translational medicine. This includes particularly the use of Solid Phase Microextraction (SPME) for metabolomic and lipidomic analysis towards biomarkers discovery, spatial and temporal resolution analysis of organs in vivo and coupling SPME to analytical instrumentation to provide clinicians with rapid diagnostic tool for real time monitoring in surgery and emergency room. Another goal is to build analytical platform for monitoring drugs and determining metabolome changes induced by pharmacotherapy, which could be used for personalized medicine. Dr. Bojko’s research team also works on application of uniform microextraction-based protocol for metabolomic and lipidomic study at subsequent steps of drug discovery and development: from high throughput format compatible with routinely used 2D and 3D cell line culturing assays to minimum invasive in vivo samplings of laboratory animals.

Scientific achievements and recognitions
Dr. Bojko is the author of 81 articles in peer-reviewed scientific journals and 3 chapters in books with a total of 1709 citations (1451 without self-citations), her h-index is 25 (SCOPUS, 30/09/2018). She received awards and distinctions for her scientific and organizational work, including those from the Minister of Health (2011, 2014), Rector of the Medical University of Silesia (2012, 2010, 2009, 2008), Medical University of Gdańsk (2014) and Nicolaus Copernicus University (2014, 2016) and a diploma from the Mayor of the City of Bydgoszcz (2016).