Fundamental and practical aspects of metabolomics: experimental design, methods and data analysis

15 September
10:00 – 12:00 (2 hours)
Provided by: Dr. Rawi Ramautar and Dr. Oleg Mayboroda, Leiden University, the Netherlands

The course is designed to give an introduction in metabolomics in a nutshell format. The first, analytical part of the course focuses on two analytical techniques, namely capillary electrophoresis-mass spectrometry (CE-MS) and nuclear magnetic resonance (NMR) spectroscopy. Using carefully pre-selected set of real-life examples we explain the basics of the experimental design, rules for the selections of optimal analytical conditions for a given task (e.g. CE separation modes, capillary coatings, interfacing designs for hyphenating CE to MS or the pulse sequences for NMR spectroscopy). In the second part, we provide guidelines for the data analysis which includes an overview of the main multivariate modeling methods, the approaches for combination of the univariate and multivariate methods. In addition, we will inform you about the available open and commercial software solutions.

Rawi RamautarDr. Rawi Ramautar
Rawi Ramautar obtained his PhD on the development of capillary electrophoresis-mass spectrometry methods for metabolomics from Utrecht University, the Netherlands, in 2010. Intrigued by metabolomics for disease prediction and diagnosis, Rawi switched to the Leiden University Medical Center to broaden his horizon on this topic. In 2013 and 2017, he received the prestigious Veni and Vidi research grants from the Netherlands Organization for Scientific Research for the development of CE-MS approaches for volume-restricted metabolomics. Currently, he is a principal investigator at the Leiden University where his group is developing microscale analytical workflows for sample-restricted biomedical problems. Rawi Ramautar was recently selected for the Top 40 under 40 Power List of the Analytical Scientist.

Oleg MayborodaDr. Oleg Mayboroda
PhD, associate professor, graduated from the University of St. Petersburg (St. Petersburg, Russian Federation) and obtained his PhD from the Institute of Evolutional Physiology and Biochemistry of the Russian Academy of Science (St. Petersburg, Russian Federation) in 1992. After completing his post-doctoral trainings at the Technische Universität Carolo-Wilhelmina of Braunschweig and the University of Magdeburg he moved to The Netherlands where he joined the Department of Parasitology, currently the Center of Proteomics and Metabolomics, of the Leiden University Medical Center. His current interests are the application of metabolomics in epidemiological studies as well as data analysis.